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. 2014 Jul 4;5(17):7471–7485. doi: 10.18632/oncotarget.2166

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Copyright: © 2014 Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. Comparable cytotoxicity of Lipo-DS/Cu in monolayer-cultured cells and BCSCs was detected by MTT assay. The cells were exposed to Lipo-DS/Cu for 72 hours. B. Lipo-DS/Cu induced Bax and cleaved PARP and reduced Bcl2 expression were detected by western blot. The cells were treated in Lipo-DS (1μM)/CuCl₂(10μM) for 4 hours and in drug-free free stem cell culture medium for 24 hours. C. Lipo-DS/Cu abolished sphere-forming ability in BC cell lines. The cells were cultured in Lipo-DS (1μM), CuCl₂ (10μM) or Lipo-DS/CuCl₂ for 4 hours and in drug-free free stem cell culture medium for 7 days. D. The effect of different treatments on CD24^low/CD44^high population. E. The effect of different treatments on ALDH activity in BCSCs. For experiments D and E, 7-day-cultured sphere cells were trypsinized and exposed to different agents for 4 hours, then released for 24 hours. (** in D and E: In comparison with other groups, p<0.01) F. Expression of ALDH mRNAs in attached and spheroid cells. G. Lipo-DS/Cu did not influence the expression of ALDH proteins in CSCs. The whole proteins were extracted from the BCSCs exposed to different agents for 4 hours and released in drug-free medium for 24 hours.

A. Comparable cytotoxicity of Lipo-DS/Cu in monolayer-cultured cells and BCSCs was detected by MTT assay. The cells were exposed to Lipo-DS/Cu for 72 hours. B. Lipo-DS/Cu induced Bax and cleaved PARP and reduced Bcl2 expression were detected by western blot. The cells were treated in Lipo-DS (1μM)/CuCl₂(10μM) for 4 hours and in drug-free free stem cell culture medium for 24 hours. C. Lipo-DS/Cu abolished sphere-forming ability in BC cell lines. The cells were cultured in Lipo-DS (1μM), CuCl₂ (10μM) or Lipo-DS/CuCl₂ for 4 hours and in drug-free free stem cell culture medium for 7 days. D. The effect of different treatments on CD24^low/CD44^high population. E. The effect of different treatments on ALDH activity in BCSCs. For experiments D and E, 7-day-cultured sphere cells were trypsinized and exposed to different agents for 4 hours, then released for 24 hours. (** in D and E: In comparison with other groups, p<0.01) F. Expression of ALDH mRNAs in attached and spheroid cells. G. Lipo-DS/Cu did not influence the expression of ALDH proteins in CSCs. The whole proteins were extracted from the BCSCs exposed to different agents for 4 hours and released in drug-free medium for 24 hours.