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. 2014 Jul 31;5(17):7625–7634. doi: 10.18632/oncotarget.2283

Figure 3. Endogenous MagNuM currents are mediated by TRPM7 and TRPM6.

Figure 3

Currents were measured in divalent-free internal solution with 5 mM EGTA and 5 mM EDTA. A-B, siRNAs efficacy measured by qRT-PCR analysis of TRPM6 (A) and TRPM7 (B) expression levels in the SHEP-21N cell line under both control and N-Myc upregulation conditions (n = 9). C, MagNuM currents in control SHEP-21N cells treated with negative non-silencing (siCTL) or specific siRNA sequences against TRPM7, TRPM6, TRPM7&TRPM6. D, current measurement in N-Myc-expressing SHEP-21N cells treated with negative (siCTL) or specific siRNA sequences against TRPM7, TRPM6, TRPM7&TRPM6. E, statistical summary of current amplitudes at 700 s as in c-d (*, p<0.01). The number of patched cells is indicated in graph. F, typical current traces evoked by voltage ramps (I-V curves) in N-Myc-expressing SHEP-21N cells as in D.