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. 2014 Aug 4;5(17):7788–7804. doi: 10.18632/oncotarget.2307

Figure 4. Calpain-10 regulates AhR expression in Biseugenol-treated gastric cancer cells.

Figure 4

(A). Calpain activity was measured with the fluorescent calpain substrate Suc-LLVY-AMC in N87, AGS, MKN45, and SCM-1 cells. (A) Time course responses to Biseugenol (Eug) (40 μM in N87 and AGS,; 80 μM in MKN45 and SCM-1) treatment. Data are expressed in terms of fold of control conditions. (B) Transfection with shCalpain-10 but not shcalpain-1, shcalpain-2 or scramble reduces the increased calpain activity in AGS cells treated with Eug for 8 h. Calpain inhibitor Z-Leu-Leu-CHO; 25 μM) significantly inhibited Eug-increased calpain activity. Data are presented as mean±SEM (n = 5). (C) AGS and SCM-1 cancer cells were treated with Eug either in a dose- or time-dependent manner, and then AhR and Arnt expressions were evaluated by Western blotting. (D) The expressions of AhR or Calpain-10 in gastric cancer cells (AGS and SCM-1) with or without Biseugenol (AGS, 40 μM or SCM-1 80 μM) treatment for 24 h in the presence or absence of a Calpain inhibitors Z-Leu-Leu-CHO or Calpain-10 siRNA transfection were detected. The results shown are representative of at least four independent experiments.