Skip to main content
. 2014 Aug 4;5(17):7833–7842. doi: 10.18632/oncotarget.2312

Figure 6. Stem cell signalling inhibitors decrease self renewal of MCF7 stem-like cells.

Figure 6

Breast cell line (MCF7) were live cell sorted by FACS. Cells were sorted by ESA +/−, then CD44+/−, then CD24 lo/high to gain CD44+/24lo/ESA+ (Stem-like cells) and CD44-/24hi/ESA- (Non stem-like cells). These populations were sorted according to cell cycle (Hoechst DNA profile) into G0/1 and S/G2/M, and for comparison the whole cell population (all) and mammosphere formation assayed +/− DKK1 (50ng/ml) or +/− DBZ (10 μM) (Black bars). After five days of growth MS were collected and secondary MS plated, and counted after a further 5 days. Bar charts represent the % secondary mammosphere formation; ±SEM. p values were generated using a two sided t-test assuming equal variance. * indicates significance, p<0.05