Fig. 8.—

Multiple sequence alignment of gigantoxin domain sequences generated by MAFFT-L-INS-i (Katoh and Standley 2013). The signal peptide, propeptide, and domain regions of the cnidarian sequences and the two Glycera peptides that most strongly resemble them are highlighted in colored boxes. Glycera contig 117671minus1 has a leucine residue in position 91 of the alignment, which is thought to be involved in mediating the high-affinity binding of the EGF domain to the TRPV1 receptor, triggering the associated pain pathway.