Table 2.
Effects of Sargassum polycystum and Padina tenuis extracts and fractions on mushroom tyrosinase inhibition assay.
| Extracts a | Mushroom tyrosinase activity inhibition (%) b | ||||||||
| 0µg/ml | 10µg/ml | 30µg/ml | 50µg/ml | 70µg/ml | 100µg/ml | 250µg/ml | 500µg/ml | 1000µg/ml | |
| Kojic Acid c | 0.00 | - | - | - | - | 87.15±1.25** | - | - | - |
| SPEt | 0.00 | 5.19±4.60 | 4.81±4.55 | 4.08±3.48 | 6.30±3.97 | 7.06±3.55* | 9.83±2.12** | 11.89±1.88** | 11.63±1.80** |
| SPHF | 0.00 | 3.26±2.71* | 1.08±4.83 | 1.08±4.50 | 5.57±3.14** | 3.60±3.65 | 10.14±8.43* | 3.12±5.19 | −19.34±16.92* |
| SPEA | 0.00 | 4.73±1.61** | 2.47±2.30 | 2.68±0.97* | 5.44±2.22* | 9.63±12.13 | 6.54±1.16** | 2.32±3.44 | 18.72±11.23* |
| SPWF | 0.00 | 7.38±3.32* | 8.50±1.92** | 7.40±2.22** | 7.85±2.33** | 8.24±4.35* | 0.95±7.38 | 0.77±7.74 | 1.79±6.57 |
| PTEt | 0.00 | 6.13±3.42** | 7.31±2.55*** | 7.34±3.17** | 10.07±3.93** | 8.98±3.99** | 11.49±2.37*** | 9.98±3.84** | 11.91±6.54** |
| PTHF | 0.00 | 15.94±4.21** | 14.99±5.68* | 16.34±1.96*** | 18.44±3.28** | 17.30±1.05*** | 9.47±5.56* | −15.95±4.35** | −62.39±12.27** |
| PTEA | 0.00 | 8.36±2.95* | 6.91±2.83* | 9.39±2.82** | 1.14±1.86 | −0.07±5.92 | 2.48±3.05 | −0.71±7.94 | −17.09±20.59 |
| PTWF | 0.00 | 4.47±2.06** | 2.06±2.51 | 3.86±3.03** | 4.87±2.14** | 5.41±6.49 | 6.57±2.07** | 7.19±1.52** | 6.19±3.50* |
Data are presented as mean ± SD of 4 independent experiments. *p<0.05, **p <0.01, ***p<0.001.
SPEt: Sargassum polycystum Ethanol crude extract; SPHF: S. polycystum Hexane fraction; SPEA: S. polycystum Ethyl Acetate fraction; SPWF: S. polycystum Water fraction; PTEt: Padina tenuis Ethanol crude extract; PTHF: P. tenuis Hexane fraction; PTEA: P. tenuis Ethyl Acetate fraction; PTWF: P. tenuis Water fraction.
Activity (%) = Percentage of sample versus control.
Kojic acid was used as positive control at the concentration of 100µg/ml.