Table 3.

Effects of Sargassum polycystum and Padina tenuis extracts and fractions on cell viability assays in HEM cells. Control wells without treatment were set as 100% and results were expressed as percentage to their respective controls

Extracts a	Cytotoxicity in HEM cells (%) b						IC50 (µg/ml) c
	0µg/ml	10µg/ml	30µg/ml	50µg/ml	70µg/ml	100µg/ml
SPEt	0.00	30.2±3.65**	47.6±10.00*	54.0±15.61*	72.5±1.21***	77.4±3.79***	36
SPHF	0.00	−7.4±9.14	3.2±10.15	30.6±6.09*	55.3±3.47**	75.8±17.51*	65
SPEA	0.00	3.1±5.05	18.1±1.80**	26.0±18.08	31.8±10.62*	18.3±13.56	>100
SPWF	0.00	31.1±12.51	15.8±14.34	1.4±14.45	22.1±7.70*	10.5±17.24	>100
PTEt	0.00	−9.0±4.98	−1.4±4.06	0.2±20.58	19.7±8.24	44.0±12.29*	>100
PTHF	0.00	18.9±19.56	38.4±4.09**	41.5±7.76*	47.3±9.69*	60.5±5.97**	76.5
PTEA	0.00	2.5±19.84	13.1±17.85	8.7±8.03	21.9±4.04*	20.8±19.72	>100
PTWF	0.00	−4.6±9.28	4.7±6.90	2.0±8.16	13.3±15.92	5.1±17.74	>100

Data are presented as mean ± SD of 3 independent experiments. *p <0.05, **p <0.01, ***p<0.001.

^aSPEt: Sargassum polycystum Ethanol crude extract; SPHF: S. polycystum Hexane fraction; SPEA: S. polycystum Ethyl Acetate fraction; SPWF: S. polycystum Water fraction; PTEt: Padina tenuis Ethanol crude extract; PTHF: P. tenuis Hexane fraction; PTEA: P. tenuis Ethyl Acetate fraction; PTWF: P. tenuis Water fraction.

^bActivity (%) = Percentage of sample versus control.

^cIC₅₀ (the concentration which will exert 50% inhibitory effect) was calculated based on the graph plotted using Microsoft Excel 2013.