Table 4.

Effects of Sargassum polycystum and Padina tenuis extracts and fractions on cell viability assays in Chang cells. Control wells without treatment were set as 100% and results were expressed as percentage to their respective controls

Extracts a	Cytotoxicity in Chang cells (%) b						IC50 (µg/ml) c
	0µg/ml	10µg/ml	30µg/ml	50µg/ml	70µg/ml	100µg/ml
SPEt	0.0	9.1±9.98	21.8±2.85**	41.7±7.55*	54.7±3.34**	61.0±3.33**	61
SPHF	0.0	−3.9±2.69	−0.6±3.80	5.1±2.98	20.2±8.23	76.8±13.96*	86.5
SPEA	0.0	−12.3±4.88*	−11.5±3.46*	−0.5±6.86	10.2±5.53	24.0±8.92*	>100
SPWF	0.0	11.9±2.35*	9.0±1.62*	14.0±1.24**	14.4±1.80**	19.1±0.14***	>100
PTEt	0.0	16.4±4.84*	50.1±1.65***	52.2±2.64***	48.4±2.84**	58.7±1.93***	30
PTHF	0.0	−6.0±6.42	4.5±10.59	16.3±5.07**	14.1±7.33	39.3±5.87**	>100
PTEA	0.0	3.1±3.57	17.8±6.40*	26.8±6.86*	40.8±7.77*	58.6±1.06***	85
PTWF	0.0	2.6±4.21	5.5±6.43	8.9±2.38*	9.5±6.86	12.0±3.42*	>100

Data are presented as mean ± SD of 3 independent experiments. *p <0.05, **p <0.01, ***p<0.001.

^aSPEt: Sargassum polycystum Ethanol crude extract; SPHF: S. polycystum Hexane fraction; SPEA: S. polycystum Ethyl Acetate fraction; SPWF: S. polycystum Water fraction; PTEt: Padina tenuis Ethanol crude extract; PTHF: P. tenuis Hexane fraction; PTEA: P. tenuis Ethyl Acetate fraction; PTWF: P. tenuis Water fraction.

^bActivity (%) = Percentage of sample versus control.

^cIC₅₀ (the concentration which will exert 50% inhibitory effect) was calculated based on the graph plotted using Microsoft Excel 2013.