Table 2. Methods commonly used in the estimation of purity of glucan preparations.
| Analyte | Method | Basis | References |
|---|---|---|---|
| Total sugars | Anthrone or phenol—sulfuric acid assay | Colorimetry | (48) |
| Reducing sugars | Folin-Wu | Colorimetry | (49) |
| Somogyi-Nelson | (50,51) | ||
| 3,5-dinitrosalicylic acid | (52) | ||
| P-Hydroxybenzoic acid hydrazide | (53) | ||
| 2,2'-bicinchoninate | (54) | ||
| Starch, amylose | Iodine test | Colorimetry | (55) |
| Chitin | GlcN after total hydrolysis | Chromatography | (56) |
| (57) | |||
| Acetic acid after deacetylation | (58) | ||
| Uronic acids | Carbazol | Colorimetry | (59) |
| m-Hydroxybifenyl | (60) | ||
| O-acetyls | Hestrin | Colorimetry | (61) |
| Proteins | Kjeldahl | N | (62,63) |
| Lowry | Colorimetry | (62,64) | |
| Bradford | (65) | ||
| Total phenols | Folin-Ciocalteu (galic acid equivalent) | Colorimetry | (66) |