Figure 3.

Pain scores of formalin induced nociceptive behaviors (mean ±S.E.M. of 8–10 mice per group) following injection of saline (10 ml/kg) or Diclofenac measured every 3 minutes for a total 60 minutes (A) and bar chart for different doses of morphine 1.5, 3, and 6 mg/kg in the formalin test and control site (B1-B3). Columns represent the mean nociceptive score in each phase: phase 1 (minutes 1–7), inter phase (minutes 8–14), and phase 2 (minutes 15–60) (B). The mice received the drug 30 minutes before formalin injection in the right hind paw. Recording of the nociceptive behaviors began immediately after formalin injection (time 0) and continued for 60 minutes.

*P < 0.05; **P < 0.01; and ***P < 0.001 compared with saline