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. 2014 Winter;5(1):88–95.

Table 1.

Nucleotide sequences of primer sets and annealing temperature (T) were used for RT-PCR reactions.

Gene Forward Reverse Annealing Temperature Product Size (bp)
mIL-19 ATG AAG ACA CAG TGC GCG TC GTG TCA GGC TGC AGG AG 65°C 529
mIL-1β CTC CAT GAG CTT TGT ACA AGG TGC TGA TGT ACC AGT TGG GG 63°C 240
mTNF-α ATG AGC ACA GAA ACA TGA TCC GC CCA AAG TAG ACC TGC CCG GAC TC 64°C 692
mGFAP GGC GCT CAA TGC TGG CTT CA TCT GCC TCC AGC CTC AGG TT 54°C 560
mβ-actin GGG AAT GGG TCA GAA GGA CT TTT GAT GTC ACG CAC GAT TT 58-65°C 589