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The effect of nZnO (1,5,10,20 mg/kg) on latency time to pain stimulus in hot plate test: each line show the mean ± SEM for one dose in four time (every 30 min from 0 up to 90). In every time *P < 0.05, *P < 0.01,***P < 0.001 show significant differences between treatment groups and control at the same time. +P < 0.05show differences between nZnO 10 mg/kg and nZnO 1mg/kg after 60 and 90 minutes, and ##P<. 0.01 show significant difference between nZnO 5mg/kg and 1mg/kg after 90 minutes.
