Fig. 1.

Assessment of putative miR-409-3p- and miR-216b-binding sites. HEK293 cells were cotransfected with pGL3-SV40 (empty vector; white column) or a pGL3-SV40 construct containing the putative miR-409-3p- (a) and miR-216b-binding sites (b) identified in table 1 in combination with miR-409-3p, miR-216b, or control miRNA. Data are normalized to the respective empty vector controls and expressed as relative luciferase activity. Values are presented as mean ± SEM and are representative of n = 3 independent experiments from three replicate cultures. * p < 0.005 (pGL3-216b-BS3: p = 0.002; pGL3-216b-BS3: p = 0.003); ** p < 0.001 (pGL3-409-3p-BS2: p = 0.0004; pGL3-216b-BS5: p = 0.0008).