Fig. 3.

Effect of miR-409-3p and miR-216b on TrkB expression. a SHSY5Y cells were transfected with miR-409-3p, miR-216b, or control miRNA. Expression of TrkB-Shc (white column), TrkB-TK+ (black column), and TrkB-TK-(grey column) mRNA expression was measured by qPCR. Data are expressed as TrkB mRNA normalized to β-actin mRNA and presented as mean ± SEM. Values are representative of n = 3 independent experiments from three replicate cultures. b Representative Western blots of SHSY5Y cells probed with a TrkB antibody, which can detect the predicted 140-kDa protein (white column) representing the full length TrkB proteins including the TrkB-TK+ isoform as well as the 90-kDa protein (black column) representing the truncated TrkB proteins including the TrkB-TK- and TrkB-Shc isoforms. β-Actin was probed as a protein loading control. c Bands were quantified by densitometry. TrkB protein expression was normalized to β-actin protein and presented as mean ± SEM. * p = 0.03; ** p = 0.004.