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. 2014 Oct 20;5:492. doi: 10.3389/fimmu.2014.00492

Table 2.

Comparison of characterized mammalian and bacterial enzymes that catalyze a similar reaction.

Human enzyme (Accession No.) Acceptor Bacterial enzyme (Species) (Accession No.) Acceptor Identity
Core 1 β3-Gal-transferase, C1GALT1, T synthase (NP_064541, Q9NS00) GalNAcα- WbwC (ECO104) (AAK64375, Q93NP5) GalNAcα-PP-lipid 10.5
WbiP (ECO127) (YP_002329684, B7UT68) GalNAcα-R 10.6
CgtB (Cj) (ABL75151, A2I3T3) GalNAcβ-R 8.4
β4-Gal-transferase 1, B4GALT1 (P15291) GlcNAcβ- LgtB (Nm) (EJU76624, J8YER7) GlcNAcβ-R 9.3
WfeD (Sb) (ACD37055, B5L3X1) GlcNAcα-PP-R 8.6
β3-Gal-transferase 5, B3GALT5 (NP_149361, Q9Y2C3) GlcNAcβ- WbgO (ECO55) (ADD57106, D3QY14) GlcNAcβ-R 11.4
WbbD (ECO7) (AAC27537, Q03084) GlcNAcα-PP-lipid 13
β3-GlcNAc-transferase 2, B3GNT2 (AAD09764, Q9NY97) Galβ- LgtA (Nm) (AAL12840, Q93EK6) lactose 11.6
α3-Gal-transferase, ABO transferase GTB (AAD26574, Q9UQ64) Fucα2Galβ- WbnI (ECO86) (AAV80756, Q5JBG6) Fucα2Galβ-R 20.6
α4-Gal-transferase, A4GALT (AAO39150, Q540I6) Galβ- CgtD (Cj) (AAM90647, Q8KWQ9) Galβ-R 11.6
LgtC (Nm) (AAL12839, Q93EK7) Galβ-R 10.7
α3-GalNAc-transferase, ABO transferase GTA (V5ZDN8, P16442) Fucα2Galβ- BoGT56a (Bo) (4AYL_A, A7LVT2) Fucα2Galβ-R 21.1
GTA-like (Hm) (CBG40459, D3UIY4) Fucα2Galβ-R 19.7
α3-Sialyl-transferase ST3GAL1 (NP_775479, Q11201) Galβ3GalNAc- NST (Nm) (C6S6J1) Lactoseβ-benzyl 13.2
WbwA (ECO104) (AAK64371, Q93NP9) Galβ3GalNAcα-PP-lipid 13.6
α3-Sialyl-transferase, ST3GAL4 (Q11206) Galβ3- CstI (Cj) (ADA70348, D2KQ02) Galβ3-R 10.2
α6-Sialyl-transferase, ST6GAL1 (P15907) Galβ3- Pm0188 (Pm) (Q9CP67) Galβ-R 14.6
α6Sialyltransferase (Psp) (A8QYL1) Galβ-R 14.6
Poly α8-sialyl-transferase 2, ST8SIA2 (AAH10645, Q92186) sialyl-Gal- PST (Nm) (AAS90326, Q6PUE5) GD1 ganglioside 9.8
PST (EC) (B1LEB7) Sialyl-R 5.4
α2-Fuc-transferase, FUT2 (CAQ81982, A8K2L2) Galβ- WbwK (ECO86) (AAO37719, Q5JBG3) Galβ3GalNAc- 11.9
WbsJ (ECO128) (AAO37698, Q6XQ53) Galβ4-R 15.8
FutC (Hp) (ABO61750, A4L7J1) Galβ- 17.5
α3-Fuc-transferase, FUT4 (NP_002024, P22083) Galβ4GlcNAcβ- FutA (Hp) (YP_003057735, C7BXF2) Galβ4GlcNAcβ-R 10
FutB (Hp) (YP_003057467, C7BZU7) Galβ4GlcNAcβ-R 10.2
α3FUT (Hh) (Q7VFA1) (Sialyl-)Galβ4GlcNAcβ-R 10.4
α3/4-Fuc-transferase, FUT3 (P21217) Galβ4/3GlcNAcβ- FucTa (Hp) (AAF35291, Q9L8S4) Galβ4/3GlcNAcβ-R 14.5
α6-Fuc-transferase, FUT8 (AAI42959, A5PLL2) N-glycan core NodZ (Rsp) (G9ACH1) Chitobiose 7.6

The donor substrates of mammalian and bacterial enzymes that synthesize the same linkage are the same but the preferred acceptor substrate may differ. The bacterial enzyme can mimic the mammalian enzyme and can be used to synthesize mammalian glycan structures but the amino acid sequences have little sequence identities. The percentage of amino acid identity is much higher among eukaryotic GT families and between GTs with similar action and using similar acceptors in bacteria. The accession numbers in bold (Accession No.) are from Pubmed and/or UniProt database. Bo, Bacteroides ovatus; Cj, Campylobacter jejuni; EC, E. coli; Hh, Helicobacter hepaticus; Hm, Helicobacter mustelae; Hp, Helicobacter pylori; Nm, Neisseria meningitides; Pm, Pasteurella multocida; Psp, Photobacterium sp.; Rsp, Rhizobium sp.; Sb, Shigella boydii; PP-, diphosphate-.