Effects of simvastatin on the levels of phosphorylated ERK, AKT, and STAT3. FaDu cells were treated with XRT, C225, and simvastatin as described in Figure 3. (A) Western blot analysis was used to determine the levels of proteins as it is shown. α-Tubulin was included as a loading control. (B) Optical densitometry quantifications (in arbitrary units) of the protein levels are indicated as fold relative to the XRT + C225 condition; bars show SEM. Three independent lysates, each blotted in triplicate, were used. *P < .05 compared to XRT plus C225 without simvastatin.