Table 2.
Cell Proliferation Assays in FaDu and A431 Cell Lines
| Cell Number (106) |
||||||
|---|---|---|---|---|---|---|
| 24 Hours | P Value | 48 Hours | P Value | 72 Hours | P Value | |
| FaDu cell line | ||||||
| Nontreatment | 2.63 ± 0.24 | – | 4.39 ± 0.35 | – | 5.88 ± 0.55 | – |
| XRT (3 Gy) | 1.46 ± 0.50 | .062 (1) | 2.36 ± 0.26 | .007 (1) | 2.93 ± 0.33 | .017 (1) |
| C225 (10 nM) | 2.32 ± 0.14 | .156 (1) | 3.48 ± 0.34 | .121 (1) | 4.26 ± 0.28 | .071 (1) |
| Simvastatin (15 μM) | 2.34 ± 0.60 | .497 (1) | 3.05 ± 0.35 | .042 (1) | 3.09 ± 0.42 | .017 (1) |
| C225 + simvastatin | 1.24 ± 0.29 | .027 (1) | 1.63 ± 0.31 | .007 (1) | 2.02 ± 0.50 | .007 (1) |
| XRT + C225 | 2.11 ± 0.11 | .093 (1) | 2.62 ± 0.12 | .003 (1) | 2.94 ± 0.08 | .002 (1) |
| XRT + C225 + simvastatin | 1.97 ± 0.11 | .071 (1) | 2.10 ± 0.14 | .002 (1) | 1.52 ± 0.09 | .002 (1) |
| .631 (2) | .025 (2) | .004 (2) | ||||
| A431 cell line | ||||||
| Nontreatment | 1.89 ± 0.33 | – | 2.19 ± 0.15 | – | 2.97 ± 0.38 | – |
| XRT (3 Gy) | 1.18 ± 0.07 | .136 (1) | 1.56 ± 0.04 | .010 (1) | 0.95 ± 0.04 | .010 (1) |
| C225 (30 nM) | 0.81 ± 0.02 | .011 (1) | 1.35 ± 0.07 | .010 (1) | 1.28 ± 0.09 | .010 (1) |
| Simvastatin (15 μM) | 1.49 ± 0.07 | .497 (1) | 1.14 ± 0.06 | .010 (1) | 0.92 ± 0.03 | .010 (1) |
| C225 + simvastatin | 1.01 ± 0.03 | .011 (1) | 0.53 ± 0.06 | .010 (1) | 0.42 ± 0.04 | .010 (1) |
| XRT + C225 | 1.29 ± 0.19 | .055 (1) | 1.07 ± 0.14 | .004 (1) | 0.98 ± 0.19 | .006 (1) |
| XRT + C225 + simvastatin | 0.92 ± 0.03 | .004 (1) | 0.34 ± 0.07 | .004 (1) | 0.23 ± 0.08 | .004 (1) |
| .150 (2) | .004 (2) | .004 (2) | ||||
Cell cultures were treated as indicated in the Materials and Methods section. Values are means ± SE of three independent experiments, with each experiment duplicated. Doses used in the combined treatments are the same as in the single treatments. P value (Mann-Whitney test): (1) compared to nontreatment condition and (2) compared to XRT + C225 without simvastatin.