Table 3.
Clonogenic Assays in FaDu and A431 Cell Lines
| Clonogenic Cell Survival (%) |
||||
|---|---|---|---|---|
| Drugs for 14 Days | P Value | Drugs for 48 Hours | P Value | |
| FaDu cell line | ||||
| Nontreatment | 100 ± 0.00 | – | 100 ± 0.00 | – |
| XRT (2 Gy) | 71.7 ± 1.37 | .002 (1) | 80.6 ± 3.47 | .002 (1) |
| C225 (10 nM) | 28.2 ± 1.92 | .002 (1) | 33.1 ± 1.31 | .002 (1) |
| Simvastatin (1 μM) | 93.1 ± 2.01 | .040 (1) | 87.3 ± 3.48 | .040 (1) |
| XRT + simvastatin | 69.7 ± 1.88 | .002 (1) | 62.1 ± 3.15 | .002 (1) |
| C225 + simvastatin | 23.0 ± 2.20 | .002 (1) | 68.0 ± 6.46 | .002 (1) |
| XRT + C225 | 22.4 ± 1.76 | .002 (1) | 47.1 ± 1.78 | .002 (1) |
| XRT + C225 + simvastatin | 13.0 ± 2.11 | .002 (1) | 33.0 ± 1.05 | .002 (1) |
| .004 (2) | .004 (2) | |||
| A431 cell line | ||||
| Nontreatment | 100 ± 0.00 | – | 100 ± 0.00 | – |
| XRT (2 Gy) | 78.5 ± 2.57 | .001 (1) | 89.8 ± 0.84 | .037 (1) |
| C225 (30 nM) | 74.3 ± 4.09 | .001 (1) | 88.5 ± 1.02 | .037 (1) |
| Simvastatin (1 μM) | 75.6 ± 3.47 | .001 (1) | 87.5 ± 1.30 | .037 (1) |
| XRT + simvastatin | 64.7 ± 2.21 | .001 (1) | 90.1 ± 1.95 | .037 (1) |
| C225 + simvastatin | 62.5 ± 3.50 | .001 (1) | 93.7 ± 0.52 | .037 (1) |
| XRT + C225 | 58.0 ± 3.46 | .001 (1) | 92.5 ± 1.14 | .037 (1) |
| XRT + C225 + simvastatin | 44.7 ± 4.27 | .001 (1) | 76.9 ± 3.63 | .037 (1) |
| .009 (2) | .050 (2) | |||
Cell cultures were treated as indicated in the Materials and Methods section. Values are means ± SE. For FaDu and A431 cells, two and four independent experiments, with each experiment duplicated, were performed for 14-day and 48-hour schemes, respectively. Doses used in the combined treatments are the same as in the single treatments. P value (Mann-Whitney test): (1) compared to nontreatment condition and (2) compared to XRT + C225 without simvastatin.