Table 1.
Immunohistochemistry panel used in the study.
| Stain | Clone | Dilution | Brand | Use |
| GFAP | GF2 | 1:50 | Dako-Cytomation | Glial differentiation |
| Vimentin | V-9 | 1:200 | Biogenes | Mesenchymal differentiation |
| Smooth muscle actin | ZMSA-5 | 1:50 | Dako-Cytomation | Muscle differentiation |
| Muscle specific actin | HHF35 | Prediluted | Dako-Cytomation | Muscle differentiation |
| H-caldesmon | h-cald | 1:200 | Thermo Scientific | Muscle differentiation |
| Neurofilament | 2F11 | 1:50 | Dako Cytomation | Neuronal differentiation |
| Chromogranin A | DAK-A3 | 1:100 | Dako Cytomation | Neuronal differentiation |
| Synapthophysin | SNP88 | 1:100 | BioGenex | Neuronal differentiation |
| PGP 9.5 | 1:50 | Dako Cytomation | Neuronal differentiation | |
| S100 | 4C4.9 | 1:50 | Neomarkers | Neural crest differentiation |
| CD56 | BL56CO4 | 1:50 | Biocare Medical | Neural crest differentiation |
| CD57 (Leu-7) | NK1 | 1:200 | Neomarkers | Neural crest differentiation |
| CD31 | MEM-05 | 1:50 | ZYMED | Blood vessels identification |
| CD34 | QBEND | 1:50 | BioGenex | Blood vessels identification |
| CD68 | KPI | 1:50 | Dako Cytomation | Pleomorphic xanthoastrocytoma |
| CD99/MIC2 | HO36-1.1 | 1:100 | Thermo Scientific | Other grade IV neoplasms* |
| FLI1 | FLI1 | Prediluted | Thermo Scientific | Other grade IV neoplasms* |
| BCL-2 | Bcl2/100/D5 | 1:50 | Novocastra | Molecular marker analyzed |
| p53 | DO.7 | 1:25 | Dako Cytomation | Molecular marker analyzed |
| Ki67 | MIB1 | 1:100 | Dako Cytomation | Molecular marker analyzed |
| EMA | E29 | 1:50 | Dako Cytomation | Epithelial cancer metastasis |
| Keratin | AE1AE3 | 1:100 | Dako Cytomation | Epithelial cancer metastasis |
* Primitive neuroectodermal tumor, medulloblastoma.