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. 2014 Nov 1;21(13):1823–1836. doi: 10.1089/ars.2013.5664

FIG. 1.

FIG. 1.

Effect of hyperoxia on ROS/RNS accumulation in NO-treated alveolar epithelial cells. DHR123 (A, C, E) and DCF (B, D, F) fluorescence in response to hyperoxia±NO 20 ppm,±L-Cys 0.1 mM in R3/1 (A, B), L2 (C, D), and A549 (E, F) cells. Data are normalized to cell number estimated using Hoechst 33342. Results are mean of 9±SEM. Effect of hyperoxia×12 h±NO±L-Cys on 3-nitrotyrosine (G), and protein carbonyls (H), normalized to cellular protein. Results are mean of 3±SEM. *p<0.05 versus corresponding air, †p<0.05 versus 60% O2, Δp<0.05 versus corresponding group without L-Cys. DCF, dichlorofluorescein; NO, nitric oxide.