FIG. 4.

LAT1 siRNA knockdown effect on LAT1 function and SNO uptake. LAT1 expression in A549, L2, and R3/1 alveolar epithelial cells 3 or 6 days after adenoviral siRNA (anti-sense, A/S) knockdown versus sense control, determined by (A) immunoblot and (B) immunofluorescence. (C) Immunoblot quantified by densitometry. Data are the mean of four independent experiments±SEM. *p<0.05 versus sense. (D) Effects of LAT1 siRNA knockdown on LAT1 mRNA normalized to L32. Data are mean of three independent experiments±SEM, *p<0.05 versus sense. (E) Effects of knockdown on Na-independent ³H-Leu uptake, and (F)¹⁴C mannitol control, mean of six independent experiments±SEM. *p<0.05 versus sense. (G) Effects of knockdown on SNO uptake in R3/1, L2, and A549 cells after NO exposure. The air-exposed controls are the same data depicted in Figure 2. Data are mean of five independent experiments±SEM. *p<0.05 versus air, ⁺p<0.05 versus sense. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars