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. 2014 Nov 1;21(13):1823–1836. doi: 10.1089/ars.2013.5664

FIG. 8.

FIG. 8.

Effects of hyperoxia on LAT1 expression in alveolar epithelial cells in vitro. Effects on LAT1 accumulation detected by immunoblot in whole cell lysates (A–F, 90 μg/lane) and plasma membrane fractions (G, H, 30 μg/lane) in R3/1, L2, and A549 cells. Representative immunoblots and densitometry for R3/1 (A, B) L2 (C, D), and A549 (E, F) cells after exposure to air or hyperoxia (60% or 90%). Densitometry analysis of LAT1 expression (80 kDa bands, gray bars; 40 kDa bands, black bars) normalized to β actin or pan-cadherin, mean of 4–5±SEM for whole cell lysates. Data for plasma membrane fractions are pooled from 4 plates. *p<0.05 versus corresponding air control.