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. 2014 Sep 9;4(9):e437. doi: 10.1038/tp.2014.77

Figure 2.

Figure 2

Glucocorticoid receptors (GR) and GR targets expression is impaired in the PFC of n-3-deficient mice. GR, MR, FKB51 and BDNF proteins expression was measured with western blot analysis in the PFC and the hippocampus of control diet (white) and n-3-deficient mice (red). n-3-deficient mice showed (a) a marked downregulation of GR expression within the PFC as compared with control diet mice (t11=5.659, ***P<0.0001, unpaired t-test, n=6–7 per group). (b) There was no significant effect of n-3 PUFA-deficient diet on either the 70-kDa MR (t12=1.274, P>0.05, unpaired t-test, n=7 per group) or the 102-kDa MR (t12=0.05060, P>0.05, unpaired t-test, n=7 per group) expression within the PFC. GR-dependent target (c) BDNF and (d) FKBP51 expression was significantly reduced in the PFC of n-3-deficient mice as compared with control diet mice (t10=3.671, **P<0.01, unpaired t-test, n=6 per group and t10=3.351, **P<0.01, unpaired t-test, n=6 per group, respectively). There was no effect of dietary n-3 PUFA deficiency on the expression of (e) GR (t11=0.1953, P>0.05, unpaired t-test, n=6–7 per group), (f) 70-kDa MR (t12=1.599, P>0.05, unpaired t-test, n=7 per group), 120-kDa MR (t10=0.8858, P>0.05, unpaired t-test, n=6 per group), (g) GR-dependent target BDNF (t11=1.097, P>0.05, unpaired t-test, n=6–7 per group) and (h) FKBP51 (t8=1.730, P>0.05, unpaired t-test, n=5 per group) in the hippocampus as compared with control diet. Data are displayed as mean±s.e.m.