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. 2014 Aug 23;65(20):5903–5918. doi: 10.1093/jxb/eru330

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — VTC2 depletion improves SE in Arabidopsis. (A) Schematic representation of the SE process in Arabidopsis. (B) Callus size significantly increases in vtc2-5 (solid line) compared with the WT [dashed line; means±standard deviation (SD)]. (C) Number of mature somatic embryos formed per callus (means±SD). Asterisks above the bar indicate significant difference from control values (P<0.05) at the same sampling time. (D) Zygotic embryos in WT (top) and vtc2-5 (bottom). Bar, 1mm. (E) Representative embryos at 7 d on induction medium. Bar, 1.5mm. (F) At 14 d on induction medium, proliferation of the embryo is much more profound in vtc2 compared with WT. Bar, 3.5mm. (G) Embryos were transferred to a medium lacking 2,4-D. Here, we show three representative clusters of somatic embryos at 14 d of maturation. Bar, 5mm. (This figure is available in colour at JXB online.)