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. 2014 Aug 28;65(20):6107–6122. doi: 10.1093/jxb/eru351

Fig. 1.

Fig. 1.

Characterizations of NbNHX1. (A) The alignment of the deduced amino acid sequences of NbNHX1, SeNHX1, and AtNHX1. The box indicates a putative amiloride binding site. NHX1 proteins contain 12 transmembrane domains which are indicated as TM1–TM12. (B) Phylogenetic analysis of NbNHX1, SeNHX1, and the NHX family in Arabidopsis. The NHX family (with their TAIR accession numbers) is as follows: AtNHX2-1 (AT3G05030.1); AtNHX2-2 (AT3G05030.2); AtNHX3 (AT5G55470); AtNHX4 (AT3G06370); AtNHX5 (AT1G54370); AtNHX6 (AT1G79610). (C) Vacuolar H+ net flux in NbNHX1-silenced tobacco plants. Mean H+ net fluxes for a period of 12min. (D, E) Mean H+ net fluxes in vacuoles supplied with 0, 25, and 50mM NaCl (D) or KCl (E). The value obtained from NMT indicates net ion flux, and the positive values of ion flux in the figures represent cation efflux from the vacuole into the cytoplast, and vice versa. Data are means ±SE (n = 36 vacuoles from six independent NbNHX1-silenced lines). The asterisks on the bars indicate significant differences from the TRV plants (C) or untreated plants (D and E) in the same treatment at P≤0.05.