Fig. 4.

Disease development in Se/AtNHX1 transgenic plants after Ppn inoculation. (A) Disease symptoms on tobacco leaves at 0 and 72 hpi. Wounding-only treatment on the left side of the leaf served as a control (scale bar=2cm). S3/12/25 and A2/14/28 represent tobacco seedlings with different expression of NHX1 based on Supplementary Fig. S2G at JXB online. (B–D) Area of wilt spots (B), development of disease course (C), and H₂O₂ content (D). (E, F) Correlation analysis between NHX1 expression and wilt spot area in SeNHX1 transgenic tobacco (E) and AtNHX1 transgenic tobacco (F). YFP indicates pCAPE2-YFP vector-transformed tobacco plants as control and Se/At-YFP indicates pCAPE2-Se/AtNHX1-YFP vector-transformed tobacco plants. Data are means ±SE (n=30 leaves from 30 independent SeNHX1 and AtNHX1 transgenic lines, respectively). The asterisks on the bars indicate significant differences from the YFP plants in the same treatment at P≤0.05. (This figure is available in colour at JXB online.)