Fig. 7.

Flow cytometric analysis of the cellular oxidation level in GFP and Nb-GFP tobacco plants. (A–C) Flow cytometric analysis on protoplasts with fluorescence in control plants (A), GFP plants (B), and Nb-GFP plants (C). Biparametric outputs are displayed by the intensity of fluorescence and FSC (forward and side scatter values). The select boxes R6 or R7 are set so that near zero levels (<1%) of control protoplasts show fluorescence. (D, E) Protoplast without DR treatment (D), or with DR treatment (E). (F, G) Protoplasts screened by R6 are analysed on the DR fluorescence level by R7. (H) Average fluorescence intensity of the protoplasts in (F) and (G) screened by R7. Data are means ±SE (n=6 batches of protoplasts from six independent NbNHX1-overexpressing lines). The asterisks on the bars indicate significant differences from the GFP plants in the same treatment at P≤0.05. (This figure is available in colour at JXB online.)