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. 2014 Aug 15;7(9):5655–5664.

Figure 1.

Figure 1

Analysis of EgG1Y162 gene and protein expression. A. Relative expression level of egG1Y162 gene in developmental stages of Echinococcus granulosus. Expression of egG1Y162 gene was analyzed by fluorescent quantitative RT-PCR. Housekeeping gene egAct II was used as an internal control. B. Results of EgG1Y162 recombinant protein expression by SDS-PAGE electrophoresis analysis. Expression of pET-41a/EgG1Y162 plasmid was induced by IPTG (final concentration of 0.5 mmol/L) for 0 h, 2 h, 4 h, 6 h, respectively, at 30°C. C. Results of purified EgG1Y162 recombinant protein by SDS-PAGE electrophoresis analysis. EgG1Y162 recombinant protein was eluted with imidazole at concentrations of 0, 100, 200, 300, 500 mmol/L (pH 7.4).