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. 2014 Aug 15;7(9):5683–5691.

Figure 5.

Figure 5

miR-423-3p target validations. A. Illustration of the construction of pmiR-report-AdipoR2 UTR and -DUSP4 UTR vectors. B. Relative luciferase activity after co-transfection with either pmiR-AdipoR2 UTR/pmiR-pmiR-AdipoR2 Mutant vectors or pmiR-DUSP4 UTR/pmiR-DUSP4 UTR Mutant vectors (100 ng for each vectors); scramble and antimiR-423-3p (40 nM), 48 hours post-transfection. Both experiments were performed on Hep-2 cells. C. Western blotting of AdipoR2 in Hep-2 cells was determined 72 hours post-transfection.