Figure 2.

Kncokdown of PTPRU inhibits growth of gastric cancer cells. (A) AGS and SGC7901 cells expressing scrambled shRNA (Control) or PTPRU shRNA (shPTPRU) were seeded onto 96-well plates in quintuplicate, and proliferation rates were measured by MTT assay. (B) The colony formation ability of AGS and SGC7901 cells from each treatment group was measured by colony formation assay. Quantification of colonies in each group is shown on the right panel. (C) The cell cycle distribution of AGS and SGC7901 cells from each treatment group was analyzed by flow cytometry. The number of cells in G0/G1, S and G2/M phases are shown (n = 3). (D-F) Levels of various proteins in AGS and SGC7901 cells from each treatment group were assessed by western blot. GAPDH (D, E) and Histone H3 (F) were used as the loading control. *P < 0.05, **P < 0.01, ***P < 0.001.