Figure 3.

Knockdown of PTPRU inhibits migration, invasion and adhesion of gastric cancer cells. (A, B) Confluent monolayers of AGS and SGC7901 cells were wounded and edges of each wound were imaged right after wounding (0 h) and 24 h later. The black dotted line represents the initial wound edge. Cell migration distances in 24 h were measured using Image J software and shown in (B). (C, D) Transwell chambers without matrigel were used to assess the migratory ability of AGS and SGC7901 cells. The number of migrating cells, averaged over nine randomly selected fields of view, is quantified in (D). (E, F) Matrigel invasion chambers were used to assess the invasiveness of AGS and SGC7901 cells. The number of invading cells was counted and averaged over nine randomly selected fields, as showed in (F). (G, H) Cell-matrix adhesion assay was used to assess the adhesion ability of AGS and SGC7901 cells. The number of adherent cells, averaged over three random selected fields, is showed in (H). (I) Levels of focal adhesion proteins and VE-cadherin were detected by western blot. GAPDH was used as the loading control. Magnification, ×100 for all pictures; scale bar, 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001.