Figure 4.

Effect of isoflurane anesthesia on ERK1/2 activation in the hippocampus of mouse brain. Animals were terminated at 24 hours post-anesthesia (A) and 2 weeks post-anesthesia (B) for antibodies against phospho-ERK1/2 and ERK1/2, followed by quantification of the ratio of phospho-ERK over total ERK to determine ERK activation. A. I₁ and I₂ groups increased ERK activation significantly compared with control group (P < 0.01), I₃ and I₄ groups decreased ERK activation compared with control group (P < 0.05). B. At 2 weeks post anesthesia, I₃ and I₄ decreased ERK activation continuously (P < 0.05). There were no difference between group I₁, I₂ and control group. [Data are presented as means, error bars represent SD. One-way ANOVA and LSD analysis were performed, *p < 0.05, **p < 0.01].