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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: J Immunol. 2014 Jun 13;193(2):529–539. doi: 10.4049/jimmunol.1303247

FIGURE 5.

FIGURE 5

Papain immunization increases expression of MHC-II and CD86 on PLN B cells in a STAT6 and IL-4 dependent manner. (A) Time course of B cell MHC-II MFI in naïve mice and in papain immunized mice 3–5 d post-papain immunization and representative histogram showing B cell MHC-II expression in naïve animals (black) and in mice immunizated with papain 5 d prior (red) compared to isotype control staining (grey). Points indicate mean ± SD and data was obtained from 2 independent experiments with n=3 mice per group per experiment, ANOVA p<0.0001. (B) Time course of B cell CD86 MFI in naïve mice and in mice at d 3–5 post-papain immunization and representative histogram showing B cell CD86 expression in naïve animals (black) and in mice immunized with papain 5 d prior (red) compared to isotype control staining (grey). Points indicate mean ± SD and data was obtained from 2 independent experiments with n=3 mice per group per experiment, ANOVA p=0.0001. (C) Representative histograms indicating B cell expression of CD86 (left panel) and MHC-II (right panel) in naïve animals (black) and in WT (red) and STAT6−/− (blue) mice 5 d post-papain immunization compared to isotype control staining (grey). (D) Representative histograms indicating B cell expression of CD86 (left panel) and MHC-II (right panel) in naïve animals (black) and in WT (red) and IL-4−/− (blue) mice 5 d post-papain immunization compared to isotype control staining (grey).