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Physical map of the pSUPER-β6 construct. (A) Schematic diagram of siRNA targeting β6 gene expression vector pSUPER-β6. The chemically synthesized stem-loop oligonucleotide was subcloned into pSUPER between BglII/HindIII restriction enzyme sites. The expression of β6 siRNA with 19-bp stem and 8-nt loop was driven by Polymerase III-dependent H1 RNA promoter. (B) Sequences of β6-shRNAs.
