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. 2014 Aug 20;32(5):1787–1795. doi: 10.3892/or.2014.3419

Figure 2.

Figure 2

Analysis of shRNA-mediated silencing of ανβ6 mRNA expression in MCF-7 cells. (A) Semi-quantitative RT-PCR showed that the ανβ6 mRNA levels were significantly reduced in the MCF-7/ανβ6-1 and MCF-7/ανβ6-2 cells compared to the level in the MCF-7/CON cells. MCF-7 cells were stably transfected with recombinant plasmid pSUPER-β6shRNA1, pSUPER-β6shRNA2 and parental vector pSUPER.retro, respectively. ανβ6 and GAPDH mRNA levels were then determined by RT-PCR. GAPDH was used as an internal loading control. (B) Relative ανβ6 mRNA levels were normalized against GAPDH expression in the untreated MCF-7 and treated cells (MCF-7/CON, MCF-7/ανβ6-1 and MCF-7/ανβ6-2 cells). Error bars indicate standard deviations. *P<0.05, **P<0.01 and ***P<0.001 vs. MCF-7/CON control cells. The ratio of ανβ6 to GAPDH is shown on the y-axis. The data presented are representative of at least three independent experiments.