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. 2014 Aug 20;32(5):1787–1795. doi: 10.3892/or.2014.3419

Figure 3.

Figure 3

Charaterization of the shRNA-mediated decrease in the ανβ6 protein content in MCF-7 cells. (A) Western blotting showed obviously reduced protein expression of ανβ6 in the untreated MCF-7 and treated cells (MCF-7/CON, MCF-7/ανβ6-1 and MCF-7/ανβ6-2 cells). MCF-7 cells were transfected with β6-shRNA expressing plasmids for 72 h. Cell lysates (50 μg) were then subjected to western blot analysis with the anti-ανβ6 monoclonal antibody 10D5. GAPDH levels were detected as a loading control using the anti-GAPDH monoclonal antibody. (B) Relative ανβ6 protein levels were normalized to GAPDH in the MCF-7/ανβ6-1 and MCF-7/ανβ6-2 cells compared to the level in the MCF-7/CON cells. The error bars indicate standard deviations. Differences in the ανβ6 protein levels are statistically significant: *P<0.05, **P<0.01 and ***P<0.001, vs. the control cells. Data are from a single experiment, indicative of at least three independent experiments.