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. 2014 Aug 20;32(5):1787–1795. doi: 10.3892/or.2014.3419

Figure 6.

Figure 6

Effects of β6-shRNA on uPA expression. (A) Silencing of integrin ανβ6 inhibited the secretion of uPA in tumor conditioned medium from MCF-7 cells. MCF-7 cells were treated with pSUPER-β6shRNA1, pSUPER-β6shRNA2 or pSUPER-control for 72 h and TCM was prepared. A protein band of ~55 kDa was monitored by western blotting. GAPDH was also determined as a control for equivalent protein loading. The levels of uPA in MCF-7/ανβ6-1 and MCF-7/ανβ6-2 cells were significantly lower than that of the MCF-7/CON control cells. (B) Relative uPA expression was normalized against GAPDH level in the untreated MCF-7 and treated cells (MCF-7/CON, MCF-7/ανβ6-1 and MCF-7/ανβ6-2 cells). Error bars show standard deviations. *P<0.05, **P<0.01 and ***P<0.001 vs. the control cells. Data are indicative of three independent experiments.