Figure 2.

Plumbagin induced apoptosis of HCT116 cells via the mitochondria-mediated pathway. (A) Increased apoptosis were detected by fluorescence microscopy images (x100) of EthD-1 stained HCT116 cells after treatment for 24 h with the indicated doses of Plumbagin, added either when cells were already attached or at the time of seeding, when cells were still unattached. (B) Changes in mitochondrial transmembrane potential induced by Plumbagin in similarly treated cells were determined using the MitoCapture™ apoptosis detection kit. In all cases, DMSO treated HCT116 cells served as control.