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Generation of reactive oxygen species (ROS) in HCT116 cells after treatment with Plumbagin. Dose-dependent increase in ROS production by HCT116 cells after 24-h treatment with Plumbagin as described. ROS levels were determined using H2DCF-DA and detected fluorimetrically. Experiments were done in triplicate and results are expressed as the mean ± SD. *Significance is indicated as p<0.01.
