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. 2014 May 5;6(6):760–777. doi: 10.1002/emmm.201303626

Figure 3. Gene expression of cardiac transcription factors, cardiogenic molecules, and cardiac structural factors in endogenous cardioblasts.

Figure 3

RT-qPCR in RNA extracted from FACS-sorted GFP+ and GFP− myocyte-depleted cardiac cells revealed increased expression of cardiogenic molecules (NKX2-5, MEF2C, TBX3, FOXC2, and BMP2) and cardiac structural factors (MYH6, MYH7, MYL2, MYL3, TNNI3, TNNT2, MYBPC3, PLN, DES, NPPA, CKM, and TTN) in GFP+ cardioblasts compared to GFP− cardiac cells. Compared to adult cardiomyocytes, GFP+ cardioblasts exhibited lower expression of NKX2-5, and of all investigated cardiac structural factors (except of MYH7), similar expression of MEF2C, TBX3 and GATA4, and increased expression of BMP2 and FOXC2. Expression of fibroblast (VIM)-, endothelial (VWF)-, and smooth muscle cell (ACTA2)-related genes was downregulated in GFP+ cardioblasts (*P < 0.05 compared to GFP− cells, #P < 0.05 compared to GFP+ cells, n = 5 mice for isolation of FACS-sorted GFP+ and GFP− non-myocyte cells, n = 3 mice for adult cardiomyocytes).