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. 2014 May 5;6(6):760–777. doi: 10.1002/emmm.201303626

Figure 6. Origins of endogenous cardioblasts.

Figure 6

  1. Study schematic. Bone marrow from Myh6-MerCreMer was transplanted into lethally irradiated non-transgenic background mice. Bone marrow-reconstituted mice were randomized to undergo sham surgery or MI, followed by 4OH-tamoxifen pulsing.
  2. LacZ cells were readily detectable in the infarct region by immunohistochemistry (left) and immunocytochemistry (right), indicating successful reconstitution of the bone marrow (blue: DAPI, green: GFP, red: αSA, white: lacZ).
  3. Representative flow cytometry plots of enzymatically digested myocyte-depleted cardiac cell preparations for side scatter (SSC) and GFP expression (color gating has been applied to the images). Numbers indicate average % GFP positivity in each group.
  4. Quantification of GFP+ cardioblasts by flow cytometry (FC) and epifluorescence microscopy (epi) in sham-operated (sham) and infarcted (MI) hearts from bone marrow-reconstituted mice. Not a single GFP+ cell was detected by epifluorescence microscopy (n = 4–5 mice/group).
  5. Study schematic. Bitransgenic mice, pre-pulsed with 4OH-tamoxifen, were randomized to undergo sham surgery or MI.
  6. Representative flow cytometry plots of enzymatically digested myocyte-depleted cardiac cell preparations for side scatter (SSC) and GFP expression (color gating has been applied to the images). Numbers indicate average % GFP positivity in each group.
  7. Quantification of GFP+ cardioblasts by flow cytometry (FC) and epifluorescence microscopy (epi) in sham-operated (sham) and infarcted (MI) hearts from pre-pulsed bitransgenic mice (*P < 0.05 compared to sham, n = 3–5 mice/group).