Figure 6.
Effect of CES1 knockdown in THP-1 macrophages on cholesterol efflux and cholesteryl ester mass. (A) Efflux of [3H]-cholesterol to ApoA1 was unaltered in THP-1 macrophages transduced with lentivirus containing CES1 shRNA in either the presence or absence of LXR ligand T0901317. (B) Cholesteryl ester (CE) mass, but not free cholesterol (FC), was significantly lower in THP-1 macrophages transduced with lentivirus containing CES1 shRNA (CES1KD) compared to that in THP-1 macrophages transduced with lentivirus containing scrambled shRNA (Control). Intracellular cholesterol levels were determined at 0 h (following 24 h acLDL loading and an overnight equilibration period) and 24 h (allowing cholesterol to efflux in the presence of 10% fetal bovine serum). Data in each panel represent the mean ± SD of 3 wells; * p < 0.05, Student’s t-test.