Figure 7.

Knockdown of CES1 in THP-1 macrophages affects cholesterol uptake: Effects of CES1 silencing on scavenger receptor mRNA levels. (A, B) Total cholesterol content (FC + CE mass, A) and radioactivity ([³H]-cholesterol equivalents, B) are reduced in CES1KD THP-1 macrophages (CES1 shRNA) compared to that in control THP-1 macrophages (scrambled shRNA) after cholesterol loading with acLDL/[³H]-cholesterol. scram, scrambled. (C) Real-time PCR analyses of select genes in non-acLDL loaded (left) or acLDL loaded (right) THP-1 macrophages. mRNA expression is normalized to GAPDH mRNA, and fold differences due to CES1 silencing are expressed relative to control conditions (i.e., non-CES1 silenced cells). Data represent the mean ± SD of 3 wells;* p < 0.05, Student’s t-test.