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HPLC analysis of product formation in the reaction of adenine propenal and N-α-acetyllysine. The indicated amounts of N-α-acetyllysine (NAL) were incubated with 10 mM adenine propenal, and reactions were monitored hourly for 9–10 h. Peaks corresponding to 3 and 4 were monitored at 280 and 300 nm, respectively, by diode-array detection. Data show the mean ± SD of three determinations.
