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LC-MS analysis of the reduced reaction intermediate (6). (A) Representative LC-MS chromatogram of 6. In this example, 5 mM N-α-acetyllysine was reacted with 10 mM adenine propenal for 30 min followed by reduction with 50 mM NaCNBH3. (B) Mass spectrum of the parent ion of the reduced intermediate displaying m/z 360 with detection in negative ion mode.
