Table 1. Modified Lysine Residues within HSAa.
| molar
ratio adenine propenal:HSA |
||||||
|---|---|---|---|---|---|---|
| residue | peptide | 10:1 | 25:1 | 100:1 | 250:1 | 500:1 |
| Lys-28 (N/A) | DAHK*SEVAHR | ● | ● | ● | ● | |
| Lys-36 (12) | FK*DLGEENFK | ● | ● | ● | ||
| Lys-161 (137) | K*YLYEIAR | ● | ● | ● | ● | |
| Lys-183 (159) | HPYFYAPELLFFAK*R | ● | ● | ● | ● | |
| Lys-214 (190) | LDELRDEGK*ASSAK | ● | ● | ● | ● | ● |
| Lys-219 (195) | LDELRDEGKASSAK*QR | ● | ||||
| Lys-236 (212) | AFK*AWAVAR | ● | ● | ● | ||
| Lys-249 (225) | FPK*AEFAEVSK | ● | ● | ● | ||
| Lys-257 (233) | AEFAEVSK*LVTDLTK | ● | ● | ● | ||
| Lys-347 (323) | NYAEAK*DVFLGMFLYEYAR | ● | ● | |||
| Lys-375 (351) | LAK*TYETTLEK | ● | ● | ● | ● | ● |
| Lys-402 (378) | VFDEFK*PLVEEPQNLIK | ● | ● | ● | ● | |
| Lys-413 (389) | VFDEFKPLVEEPQNLIK* | ● | ||||
| Lys-438 (414) | K*VPQVSTPTLVEVSR | ● | ● | |||
| Lys-549 (525) | K*QTALVELVK | ● | ● | ● | ● | |
| Lys-569 (545) | EQLK*AVMDDFAAFVEK | ● | ● | |||
| Lys-598 (574) | K*LVAASQAALGL | ● | ● | ● | ||
a
HSA was treated at the indicated molar ratios of adenine propenal and analyzed for lysine residues modified with +54.0105. The presence of each modified lysine in a sample is indicated with ●. The modified lysine in each peptide sequence is indicated with an asterisk. Equivalent lysine residues within the crystal structure of HSA are provided in parentheses.