Table 6. Steady-State Kinetic Parameters for dNTP Incorporation Opposite 8-oxoG by Wild-Type and Variant hPols ι(1-445) in the Presence of 0.15 mM Mn²⁺.

pol ι(1–445)	dNTP	Km (μM)	kcat (s–1)	kcat/Km (s–1 mM–1)	finsa	relative efficiencyb
wild-type	A	2.0 ± 0.3	0.015 ± 0.001	7.5	0.83
	T	30 ± 2	0.046 ± 0.002	1.5	0.17
	G	0.90 ± 0.07	0.014 ± 0.0002	16	1.8
	C	4.2 ± 0.2	0.038 ± 0.001	9.0	1	1
Δ1–25	A	17 ± 1	0.021 ± 0.0003	1.2	0.092
	T	5.2 ± 0.3	0.028 ± 0.001	5.4	0.42
	G	0.21 ± 0.1	0.0082 ± 0.0004	39	3.0
	C	1.8 ± 0.2	0.023 ± 0.001	13	1	1.4
ΔD17	A	2.0 ± 0.2	0.017 ± 0.0003	8.5	0.77
	T	29 ± 1	0.055 ± 0.001	5.3	0.48
	G	0.53 ± 0.06	0.013 ± 0.0003	25	2.3
	C	3.5 ± 0.2	0.040 ± 0.0006	11	1	1.2
R96G	A	3.3 ± 0.3	0.0017 ± 0.00004	0.52	0.43
	T	13 ± 1	0.0046 ± 0.0001	0.35	0.29
	G	1.8 ± 0.2	0.0038 ± 0.0001	2.1	1.8
	C	8.5 ± 0.5	0.010 ± 0.0002	1.2	1	0.13
I261M	A	1.4 ± 0.2	0.015 ± 0.0004	11	1.0
	T	25 ± 3	0.026 ± 0.001	1.0	0.091
	G	0.66 ± 0.07	0.012 ± 0.0003	18	1.6
	C	3.1 ± 0.1	0.034 ± 1.0003	11	1	1.2
E276K	A	2.2 ± 0.3	0.022 ± 0.001	10	1.4
	T	24 ± 4	0.044 ± 0.003	1.8	0.26
	G	0.60 ± 0.04	0.016 ± 0.0002	27	3.9
	C	3.2 ± 0.2	0.022 ± 0.0004	6.9	1	0.77
Y374N	A	1.4 ± 0.1	0.015 ± 0.0003	11	1.0
	T	29 ± 6	0.025 ± 0.003	0.86	0.078
	G	0.72 ± 0.06	0.018 ± 0.0003	25	2.3
	C	3.6 ± 0.1	0.040 ± 0.0003	11	1	1.2

^aMisinsertion frequency, calculated by dividing k_cat/K_m for each dNTP incorporation by the k_cat/K_m for dCTP incorporation opposite 8-oxoG. All values are presented to two significant digits.

^bRelative efficiency, calculated by dividing k_cat/K_m of each pol ι(1–445) for dCTP incorporation opposite 8-oxoG by k_cat/K_m of wild-type pol ι(1–445) for dCTP incorporation opposite 8-oxoG.