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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: Cancer Res. 2014 Oct 15;74(20):5819–5831. doi: 10.1158/0008-5472.CAN-13-3527

Figure 4. Disulfiram (DSF) induces prostate cancer cell apoptosis.

Figure 4

VCaP cells were incubated with vehicle, DSF, copper alone or DSF in combination with copper. The effects of the copper chelation (BCS) as well as the antioxidants (NAC and LGR) were evaluated. Following 24 hr. drug treatments cells were harvested, stained with Annexin V-Alexa Fluor® 488 and Sytox red dead cells staining, and analyzed by flow cytometry. The populations of Annexin V−/Sytox−, Annexin V+/Sytox−, and Annexin V+/Sytox+ correspond to live cells, early apoptotic cells, and late apoptotic cells respectively. Representative plots of one set of triplicate experiments are presented.