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Quantitative PCR analysis of genomic DNA from colorectal metastases. Quantitive PCR analysis of TYMS (right curves) and LINE element control (left curves) performed on genomic DNA from colorectal cancer metastases FU-M2 (A) and FU-M4 (B) and normal (nontumor) DNA (C). (D) Differences in threshold cycle numbers between LINE element and TYMS confirm that TYMS is present at increased gene copy numbers in colorectal metastases.
