Table 1. MALDI TOF-TOF MSMS based identification of protein differentially expressed in wild-type (control) and NtGp11 in response to the treatment of 200 mM mannitol.
| SSP no.a (Spot no.) |
Th. Mr/pI Exp. Mr/pI b | Avg Fold change c | Protein (Taxonomy) | Accession number d | Mascot Score e |
Sequence Coverage (%) f |
|---|---|---|---|---|---|---|
| Stress and defense | ||||||
| 1801 (4) |
76.14/5.19 70.13/5.01 |
3.80 | Heat shock protein 70 (Spenacia oleracia) | gi|2654208 | 329 | 17 |
| 4604 (10) |
28.90/7.79 35.24/6.67 |
2.77 | Chalcone synthase (Camellia fascicularis) | gi|28565125 | 37 | 31 |
| 2601 (12) |
18.25/6.72 37.09/5.69 |
2.16 | Glutathione peroxidase (Hordeum vulgare) | gi|6179604 | 46 | 38 |
| 1402 (13) |
21.90/5.07 30.90/5.16 |
2.19 | Putative heat shock protein (Oryza sativa var Japonica) | gi|31432124 | 45 | 34 |
| 403 (14) |
36.80/6.11 28.40/4.76 |
2.55 | 1-Aminocyclopropane-1-carboxylic acid oxidase (Stellaria logipes) | gi|2293550 | 35 | 33 |
| 8002 (22) |
25.22/5.41 45.13/6.97 |
7.32 | Chalcone synthase (Leibnitzia anandria) | gi|1403057 | 44 | 25 |
| 201 (16) |
29.80/8.20 22.40/4.69 |
2.70 | Thioredoxin peroxidase (Nicotiana. tabacum) | gi|21912927 | 74 | 42 |
| 1104 (18) |
20.34/5.07 17.30/5.18 |
2.77 | Elicitor induciible protein EIG-J7 (Capsicum annum) | gi|40287496 | 74 | 15 |
| 1702 (39) |
11.20/6.35 38.43/5.43 |
2.41 | Heme oxygenase I (N. tabacum) | gi|18874688 | 37 | 43 |
| Energy metabolism | ||||||
|---|---|---|---|---|---|---|
| 4802 (5) |
51.47/5.12 54.41/6.05 |
4.32 | ATPase β subunit (Aristolochia gigantea) | gi|52082881 | 80 | 27 |
| 2101 (19) |
26.78/8.96 18.94/5.34 |
2.75 | Chloroplast ATP synthase delta subunit (N. tabacum) | gi|19787 | 95 | 41 |
| 3001 (21) |
14.59/5.18 15.21/5.83 |
2.75 | ATP synthase CF1epsilon chain (Atropa belladonna) | gi|28261723 | 80 | 54 |
| 8202 (23) |
14.54/6.40 23.36/ 6.92 |
3.00 | Putative beta4 proteasome subunit (N. tabacum) | gi|14594929 | 64 | 32 |
| 3804 (34) |
55.45/5.26 65.42/5.67 |
177.96 | ATP synthase CF1 α chain (A. belladonna) | gi|28261702 | 80 | 14 |
| 2503 (36) |
35.19/5.6 32.48/5.27 |
150.88 | Oxygen evolving complex 33kDa photosystem II (N. tabatum) | gi|30013657 | 41 | 28 |
| 9403 (41) |
33.09/9.23 35.54/8.30 |
0 | Allinase (Allium cepa) | gi|4512105 | 48 | 29 |
| Carbon metabolism | ||||||
|---|---|---|---|---|---|---|
| 8901 (1) |
51.95/6.41 99.00/6.88 |
2.92 | RUBISCO large subunit (A. belladonna) | gi|475728 | 371 | 53 |
| 5801 (8) |
48.35/5.06 45.08/6.18 |
3.49 | RUBISCO Activase II (Gossypium hirsutum) | gi|12620883 | 62 | 32 |
| 5701 (9) |
42.74/5.50 43.58/6.18 |
3.64 | RUBISCO Activase (A. thaliana) | gi|445628 | 248 | 43 |
| 7901 (2) |
51.95/6.41 100.86/6.67 |
2.54 | RUBISCO large subunit (A. belladonna) | gi|475728 | 467 | 53 |
| 3801 (3) |
20.28/7.57 75.60/5.75 |
2.31 | RUBISCO small subunit (N. tabacum) | gi|30013663 | 417 | 62 |
| 7801 (6) |
51.94/6.41 53.70/6.67 |
3.34 | RUBISCO large subunit (Solandra gradiflora) | gi|6093933 | 206 | 40 |
| 7802 (7) |
51.95/6.41 52.85/6.77 |
2.68 | RUBISCO large subunit (A. belladonna) | gi|475728 | 553 | 55 |
| 0004 (17) |
20.28/7.57 15.75/4.7 |
15.49 | RUBISCO small subunit (N. tabacum) | gi|30013663 | 89 | 28 |
| 2705 (11) |
44.46/5.71 40.63/5.62 |
2.24 | Phosphoribulokinase precursor (A. thaliana) | gi|23197622 | 59 | 9 |
| 4801 (37) |
14.55/5.59 76.00/6.00 |
1.56 | Chain S, Crystal structure of unactivated Tobacco RUBISCO with bound phosphate ion (N. tabacum) | gi|7546556 | 351 | 91 |
| 4102 (24) |
18.70/5.59 21.05/6.11 |
0.50 | Chlorophyll a/b- binding protein type-1 (Asarina barclaiana) | gi|7271945 | 75 | 15 |
| 5001 (25) |
19.55/5.39 15.21/6.22 |
0.05 | Photosystem I assembly protein Ycf3 (N. tabacum) | gi|11465956 | 46 | 28 |
| 5503 (27) |
42.62/8.82 32.05/6.19 |
0.38 | At1g49970/F2J10_5 (A. thaliana) | gi|23308343 | 50 | 33 |
| 6603 (30) |
37.49/8.87 34.33/6.43 |
0.44 | Putative PrMC3 (O. sativa var Japonica) | gi|51535276 | 38 | 10 |
| 1304 (33) |
28.40/5.68 26.31/5.21 |
0.11 | Light harvesting chlorophyll a/b-binding protein (N. sylvestris) | gi|3036951 | 41 | 21 |
| Gene regulation | ||||||
|---|---|---|---|---|---|---|
| 6204 (38) |
42.38/5.98 23.14/6.49 |
310.46 | Homeobox transcription factor KN3 (Populus balsamifera) | gi|55276122 | 43 | 21 |
| Hypothetical / unnamed and other protein | ||||||
|---|---|---|---|---|---|---|
| 1303 (20) |
28.29/5.48 26.31/5.11 |
2.34 | Unnamed protein product (N. tabacum) | gi|19829 | 153 | 62 |
| 5301 (26) |
12.26/10.02 24.51/6.2 |
0.49 | Hypothetical protein (Zea mays) | gi|40795123 | 39 | 56 |
| 5501 (28) |
11.48/10.03 34.04/6.17 |
0.03 | Hypothetical protein (O. sativa var Japonica) | gi|50253252 | 54 | 47 |
| 6501 (29) |
23.20/5.07 32.00/6.5 |
0.31 | Hypothetical protein (A. thaliana) | gi|2829909 | 46 | 36 |
| 7503 (31) |
34.14/8.53 33.07/6.8 |
0.36 | Hypothetical protein(O. sativa varjaponica) | gi|56202236 | 38 | 26 |
| 8602 (32) |
11.02/4.68 34.60/6.94 |
0.05 | Hypothetical protein (Pennisetum glaucum) | gi|33321026 | 43 | 41 |
| 6505 (42) |
28.34/8.75 32.08/6.54 |
0.50 | P0004D12.20 (O. sativa var Japonica) | gi|34906958 | 45 | 23 |
| 5805 (35) |
50.32/5.72 54.90/6.18 |
1595.7 | ARF2 (A. thaliana) | gi|12484201 | 37 | 14 |
| 302 (15) |
29.60/4.75 26.40/3.65 |
2.56 | 29kD Ribonucleoprotein (N. sylvestris) | gi|19754 | 153 | 58 |
| 8601 (43) |
80.20/10.59 36.01/6.91 |
0 | Hypothetical protein (O. sativa varjaponica) | gi|56784271 | 41 | 56 |
| 9703 (44) |
13.67/10.57 42.55/9.21 |
0 | Hypothetical protein (O. sativa varjaponica) | gi|50944661 | 44 | 38 |
Identified differentially accumulated proteins in wild-type and NtGp11 plants in response to 200 mM mannitol were categorized in stress and defense, energy metabolism, carbon metabolism, and hypothetical/ unnamed and other protein groups. aAssigned sample protein and spot number as indicated in Figure 5. bTh Mr/pI, theoretical mass of protein in kDa and pI; Exp Mr/pI, experimental mass of protein in kDa and pI. cAverage fold change represents the ratio of change of spot intensity in comparison to the wild-type. dNCBI accession number of identified protein spots. eStatistical probability of true positive identification of predicted proteins calculated by MASCOT (http://www.matrixscience.com). fSequence coverage%: percentage of predicted protein sequence covered by matched peptides.